| Product name |
Human Noggin protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Human Noggin (Gln28-Cys232) (NP_005441.1) was expressed with 6×His tag at the C-terminus |
| Applications |
His tag |
| Applications notes |
Human |
| Activity |
Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells.The ED₅₀ for this effect is <0.05 μg/mL in the presence of 50 ng/mL of recombinant Human Noggin . |
| Protein length |
The secreted Human Noggin consists of 205 amino acids and has a predicted molecular mass of 27 kDa |
| Preparation method |
E. coli |
| Purity |
>98% as determined by SDS-PAGE. |
| Alternative |
NOG, Noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A |
| Formulation |
The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits |
Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
The protein has a calculated MW of 49.11 kDa.
The protein migrates as 27 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Noggin protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve.Please avoid vortex vigorously |
| Storage instructions |
Lyophilized Human Noggin protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% BSA, 10% FBS or 5% HSA). Please prevent freeze-thaw cycles |
| Shipping |
Gel pack with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background |
Noggin is a 46.2 kDa bioactive protein, which exists as a disulfide-linked homodimer (each chain 23.1 kDa). Noggin binds members of the transforming Growth Factors-beta (TGF beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP-4), which inactivates their activities. As a extracellular antagonist of BMP proteins, Noggin involves in the development of many body tissues, including nerve tissue, muscles, and bones. In addition, Noggin is able to inhibit chondrocyte differentiation through its interaction with GDF5. |
| Alternative |
NOG, Noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A |
| Accession |
Q13253 |
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