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Human Transmembrane protease serine 3 (TMPRSS3) ELISA Kit

Human Transmembrane protease serine 3 (TMPRSS3) ELISA Kit

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Specification

Product name Human Transmembrane protease serine 3 (TMPRSS3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transmembrane protease serine 3 (TMPRSS3) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS3 in samples. An antibody specific for TMPRSS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMPRSS3; DFNB10; DFNB8; ECHOS1; TADG12; serine protease TADG12

Product Properties

Kit components • Human Transmembrane protease serine 3 microplate
• Human Transmembrane protease serine 3 standard
• Human Transmembrane protease serine 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transmembrane protease serine 3 (TMPRSS3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMPRSS3. No significant cross-reactivity or interference between Human TMPRSS3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TMPRSS4 encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellular region containing the serine protease domain. Multiple transcript variants encoding different isoforms have been found for this gene.A weak TMPRSS4 signal was detected in normal tissues of the gastrointestinal tract and in some of the urogenital tract but was not expressed in any other normal tissues. The authors suggested that TMPRSS4 may be important in processes involved in metastasis formation and tumor invasion.
Gene ID 64699
Alternative TMPRSS3; DFNB10; DFNB8; ECHOS1; TADG12; serine protease TADG12
Accession P57727

Image & description

Fig.1. Human Transmembrane protease serine 3 (TMPRSS3) Standard Curve.

Fig.1. Human Transmembrane protease serine 3 (TMPRSS3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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