| Product name | Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit employs a two-site sandwich ELISA to quantitate WWP2 in samples. An antibody specific for WWP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWWP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WWP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WWP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | WWP2; AIP2; WWp2-like; Nedd-4-like ubiquitin-protein ligase; OTTHUMP00000174895; WW domain-containing protein 2; atrophin-1 interacting protein 2 |
| Kit components | • Human NEDD4-like E3 ubiquitin-protein ligase WWP2 microplate • Human NEDD4-like E3 ubiquitin-protein ligase WWP2 standard • Human NEDD4-like E3 ubiquitin-protein ligase WWP2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human WWP2. No significant cross-reactivity or interference between Human WWP2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | WWP2 encodes a member of the NEDD4-like protein family. The family of proteins is known to possess ubiquitin-protein ligase activity. The encoded protein contains 4 tandem WW domains. The WW domain is a protein motif consisting of 35 to 40 amino acids and is characterized by 4 conserved aromatic residues. The WW domain may mediate specific protein-protein interactions. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.The deduced 870-amino acid protein has a calculated molecular mass of 98.7 kD and shares 96% identity with human WWP2. Northern blot analysis detected 2 Wwp2 transcripts, with highest expression in testis, followed by spleen, kidney, and liver. |
| Gene ID | 11060 |
| Alternative | WWP2; AIP2; WWp2-like; Nedd-4-like ubiquitin-protein ligase; OTTHUMP00000174895; WW domain-containing protein 2; atrophin-1 interacting protein 2 |
| Accession | O00308 |
Fig.1. Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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