| Product name | IPKine™ Anti-HA Magnetic IP Kit |
| Immunogen | Synthetic Peptide |
| Reactivity | Mammals, Bacteria |
| Applications | IP |
| Applications notes | Anti-YPYDVPDYA-Tag (HA-Tag) Magnetic Beads are prepared by covalently coupling Anti-HA-Tag Mouse Monoclonal Antibody to crosslinked Magnetic Beads. The Optimized Anti-HA Magnetic Beads have more efficient antigen binding capacity. This kit provides three elution methods, including competitive elution of peptide, acid elution and denatured elution. |
| Conjugate | Magnetic Beads |
| Kit components | Non-Denaturing Lysis Buffer TBS (10×) Anti-HA Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer HA Peptide (25×) SDS-PAGE Loading Buffer (5×) |
| Features & Benefits | 1.Efficient binding capacity: ≥0.6 mg HA Tag protein/mL Magnetic Beads. • 2.Can bind to various forms of tagged proteins: N-/C-. • 3.Three elution methods: competitive elution of peptide, acid elution and denatured elution. • 4.The kit contains high-quality Anti-HA magnetic beads and all validated immunoprecipitation necessary reagents. • 5.Ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules. |
| Storage instructions | Store according to the recommended storage conditions of each component, stable for 12 months. |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Figure. The immunoprecipitation effect of Anti-HA Magnetic IP Kit used for HA-Tag fusion protein. HEK293T cells were transfected with HA-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Elution Buffer; Lane 5 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Working HA Peptide. By using peptide elution and acid elution, only contained HA-Tag fusion protein, did not contain heavy and light chains of antibody.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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