| Product name | Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD7 in samples. An antibody specific for SMAD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD7 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SMAD7; CRCS3; FLJ16482; MADH7; MADH8; MAD (mothers against decapentaplegic; Drosophila) homolog 7; MAD; mothers against decapentaplegic homolog 7; Mothers against decapentaplegic; drosophila; homolog of; 7; S |
| Kit components | • Human Mothers against decapentaplegic homolog 7 microplate • Human Mothers against decapentaplegic homolog 7 standard • Human Mothers against decapentaplegic homolog 7 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD7. No significant cross-reactivity or interference between Human SMAD7 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MAD proteins, originally defined in Drosophila, are essential components of the signaling pathways of the transforming growth factor-beta receptor family. Using a differential display approach in cultured endothelial cells subjected to multiple soluble and biomechanical stimuli, Topper et al. (1997) isolated a human endothelial cell cDNA encoding MADH7, which they called SMAD7. The predicted 426-amino acid MADH7 protein lacks the C-terminal putative phosphorylation sites present in other MAD proteins, suggesting that it may be distinctly regulated. In situ hybridization and immunohistochemical analyses on human tissues showed that MADH7 is expressed predominantly in vascular endothelium. |
| Gene ID | 4092 |
| Alternative | SMAD7; CRCS3; FLJ16482; MADH7; MADH8; MAD (mothers against decapentaplegic; Drosophila) homolog 7; MAD; mothers against decapentaplegic homolog 7; Mothers against decapentaplegic; drosophila; homolog of; 7; S |
| Accession | O15105 |
Fig.1. Human Mothers against decapentaplegic homolog 7 (Smad7) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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