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Human Sialin (SLC17A5) ELISA Kit

Human Sialin (SLC17A5) ELISA Kit

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Specification

Product name Human Sialin (SLC17A5) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Sialin (SLC17A5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC17A5 in samples. An antibody specific for SLC17A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC17A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC17A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC17A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC17A5; AST; FLJ22227; FLJ23268; ISSD; NSD; SD; SIALIN; SIASD; SLD; membrane glycoprotein HP59; sialic acid storage disease; sialin; solute carrier family 17; member 5

Product Properties

Kit components • Human Sialin microplate
• Human Sialin standard
• Human Sialin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Sialin (SLC17A5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC17A5. No significant cross-reactivity or interference between Human SLC17A5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The SLC17A5 gene encodes a vesicular excitatory amino acid transporter (VEAT) with dual physiologic functions. When present in synaptic vesicles in the central nervous system, sialin is responsible for vesicular storage and subsequent exocytosis of aspartate and glutamate. When present in lysosomes, it acts as an H(+)-coupled sialic acid exporter. The isolated sequence encoded a predicted 495-residue protein with homology to members of the anion/cation symporter (ACS) family of transporters. This family contains eukaryotic inorganic anion transporters (such as Na+/phosphate cotransporters) as well as prokaryotic organic anion transporters (including H+/acid sugar symporters for hexuronate and glucarate).
Gene ID 26503
Alternative SLC17A5; AST; FLJ22227; FLJ23268; ISSD; NSD; SD; SIALIN; SIASD; SLD; membrane glycoprotein HP59; sialic acid storage disease; sialin; solute carrier family 17; member 5
Accession Q9NRA2

Image & description

Fig.1. Human Sialin (SLC17A5) Standard Curve.

Fig.1. Human Sialin (SLC17A5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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