| Product name | Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD5 in samples. An antibody specific for SMAD5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD5 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SMAD5; DKFZp781C1895; DKFZp781O1323; Dwfc; JV5-1; MADH5; MAD homolog 5; MAD; mothers against decapentaplegic homolog 5; SMA- and MAD-related protein 5; SMAD; mothers against DPP homolog 5; mothers against deca |
| Kit components | • Human Mothers against decapentaplegic homolog 5 microplate • Human Mothers against decapentaplegic homolog 5 standard • Human Mothers against decapentaplegic homolog 5 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD5. No significant cross-reactivity or interference between Human SMAD5 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SMAD5 or Mothers against decapentaplegic homolog 5 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decapentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. It may play a role in the pathway where TGFβ is an inhibitor of hematopoietic progenitor cells. |
| Gene ID | 4090 |
| Alternative | SMAD5; DKFZp781C1895; DKFZp781O1323; Dwfc; JV5-1; MADH5; MAD homolog 5; MAD; mothers against decapentaplegic homolog 5; SMA- and MAD-related protein 5; SMAD; mothers against DPP homolog 5; mothers against deca |
| Accession | Q99717 |
Fig.1. Human Mothers against decapentaplegic homolog 5 (SMAD5) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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