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Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit

Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit

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Specification

Product name Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM20 in samples. An antibody specific for TOMM20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TOMM20; RP4-597N16.2; KIAA0016; MAS20; MGC117367; MOM19; TOM20; translocase of outer mitochondrial membrane 20 homolog; translocase of outer mitochondrial membrane 20 homolog type

Product Properties

Kit components • Human Mitochondrial import receptor subunit TOM20 homolog microplate
• Human Mitochondrial import receptor subunit TOM20 homolog standard
• Human Mitochondrial import receptor subunit TOM20 homolog detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM20. No significant cross-reactivity or interference between Human TOMM20 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein import machinery of the mitochondrial outer membrane is comprised of a dynamic complex of proteins that mediates translocation of cytosolic precursor proteins into or across the membrane. Several proteins within this complex have been identified, such as Mas20p in yeast and MOM19 in Neurospora crassa . The 145-amino acid human polypeptide shares high similarity with Mas20p and MOM19 within the N-terminal region, but exhibits only weak homology to the tetratricopeptide-repeat B domain that is found in the other 2 proteins. The authors showed that human Mas20p is targeted and inserted into the outer membrane of isolated rat heart mitochondria in the N(in)-to-C(cyto) orientation.
Gene ID 9804
Alternative TOMM20; RP4-597N16.2; KIAA0016; MAS20; MGC117367; MOM19; TOM20; translocase of outer mitochondrial membrane 20 homolog; translocase of outer mitochondrial membrane 20 homolog type
Accession Q15388

Image & description

Fig.1. Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) Standard Curve.

Fig.1. Human Mitochondrial import receptor subunit TOM20 homolog (TOMM20) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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