TraKine™ Mitochondrion Staining Kit (Orange Fluorescence)

Product Information

  • Product name

    TraKine™ Mitochondrion Staining Kit (Orange Fluorescence)

  • Application notes

    Abbkine Mitochondrion Staining Kits are a set of fluorescence imaging tools to label mitochondria of live cells. This kit uses a proprietary mitochondrial orange fluorescent probe (Ex/Em=579/599 nm), which, like other probes, the kit uses a proprietary dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. MitoOrange, a hydrophobic compound, easily permeates intact living cells and can be retained after formaldehyde fixation and permeabilization. The dye is suitable for double labeling experiments, and its orange fluorescent probes is well distinguished from other green fluorescent probes. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Fig. Hela cells stained with TraKine™ Mitochondrion Staining Kit (Orange Fluorescence).

Product Properties

  • Kit components

    • MitoOrange™ (1000×) • Assay Buffer (10×)

  • Features & Benefits

    • Enables the uniform staining of mitochondrion across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells. • Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. • Proprietary MitoOrange™ (Ex/Em = 579/599 nm)-easily permeates intact living cells and can be retained after formaldehyde fixation and permeabilization, suitable for double labeling experiments.

  • Storage instructions

    Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.

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TraKine™ Mitochondrion Staining Kit (Orange Fluorescence)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTC4004

  • Size
  • Price
  • 100T $40
  • 500T $120
  • 2000T $300

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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