|Product name||TraKine™ Mitochondrion Staining Kit (Orange Fluorescence)|
|Applications notes||Abbkine Mitochondrion Staining Kits are a set of fluorescence imaging tools to label mitochondria of live cells. This kit uses a proprietary mitochondrial orange fluorescent probe (Ex/Em=579/599 nm), which, like other probes, the kit uses a proprietary dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. MitoOrange, a hydrophobic compound, easily permeates intact living cells and can be retained after formaldehyde fixation and permeabilization. The dye is suitable for double labeling experiments, and its orange fluorescent probes is well distinguished from other green fluorescent probes. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.|
|Kit components||• MitoOrange™ (1000×)
• Assay Buffer (10×)
|Features & Benefits||• Enables the uniform staining of mitochondrion across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells.
• Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.
• Proprietary MitoOrange™ (Ex/Em = 579/599 nm)-easily permeates intact living cells and can be retained after formaldehyde fixation and permeabilization, suitable for double labeling experiments.
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.|
Fig. Hela cells stained with TraKine™ Mitochondrion Staining Kit (Orange Fluorescence).