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Human Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit

Human Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit

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Specification

Product name Human Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit employs a two-site sandwich ELISA to quantitate MTTP in samples. An antibody specific for MTTP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTTP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTTP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTTP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MTTP; ABL; MGC149819; MGC149820; MTP; OTTHUMP00000161646; microsomal triglyceride transfer protein (large polypeptide; 88kDa); microsomal triglyceride transfer protein large subunit

Product Properties

Kit components • Human Microsomal triglyceride transfer protein large subunit microplate
• Human Microsomal triglyceride transfer protein large subunit standard
• Human Microsomal triglyceride transfer protein large subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Microsomal triglyceride transfer protein large subunit (MTTP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTTP. No significant cross-reactivity or interference between Human MTTP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MTTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTTP can cause abetalipoproteinemia.
Gene ID 4547
Alternative MTTP; ABL; MGC149819; MGC149820; MTP; OTTHUMP00000161646; microsomal triglyceride transfer protein (large polypeptide; 88kDa); microsomal triglyceride transfer protein large subunit
Accession P55157

Image & description

Fig.1. Human Microsomal triglyceride transfer protein large subunit (MTTP) Standard Curve.

Fig.1. Human Microsomal triglyceride transfer protein large subunit (MTTP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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