| Product name | CheKine™ Micro Total glutathione (T-GSH) Assay Kit |
| SampleType | Serum, Plasma, Animal/Plant Tissues, Whole blood, Blood cells, Cells, Bacteria |
| Alternative | Total glutathione (T-GSH) |
| Kit components | •Extraction Buffer •Assay Buffer •GR •GR Cofactor •Chromogen •GSH Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Calibration range | 0.78-50 μM |
| Limit of detection | 0.78 μM |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at -20°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The total Glutathione has two forms: reduced form (GSH) and oxidized form (GSSG). GSSG also known as Dithioglutathione, is formed by the oxidation of two molecules of glutathione. GSSG will be reduced to GSH by glutathione reductase (GR), therefore most of glutathione exists in the reduced form. Determination of intracellular GSH and GSSG content and GSH/GSSG ratio can well reflect the redox state of cells, and is also one of the main indicators of glutathione redox cycle. CheKine™Micro Total glutathione (T-GSH) Assay Kit provides a simple, convenient and rapid method for the detection of T-GSH content, which is suitable for serum, plasma, animal and plant tissues, whole blood, blood cells, cells, bacteria, etc. sample. This kit operates on the principle of utilizing glutathione reductase (GR) to reduce GSSG to GSH, which then reacts with 5,5-disulfobis(2-nitrobenzoic acid) (DTNB) to form 2-nitro-5-mercaptobenzoic acid. The reaction products exhibits a yellow color, by measuring the change in absorbance at 412 nm, the GSH content in the sample can be determined. |
| Alternative | Total glutathione (T-GSH) |
Fig.CheKine™ Micro Total glutathione (T-GSH) Assay Kit
Author：SH Lee, YB Jang, Y Choi, Y Lee, BN Shin Publication name：Microbiology Spectrum IF：4
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