| Product name | Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit employs a two-site sandwich ELISA to quantitate MSRB2 in samples. An antibody specific for MSRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMSRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MSRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MSRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MSRB2; CBS-1; CBS1; CGI-131; MGC26104; MSRB; PILB; methionine-r-sulfoxide reductase B2 variant 1; methionine-r-sulfoxide reductase B2 variant 3; pilin-like transcription factor |
| Kit components | • Human Methionine-R-sulfoxide reductase B2, mitochondrial microplate • Human Methionine-R-sulfoxide reductase B2, mitochondrial standard • Human Methionine-R-sulfoxide reductase B2, mitochondrial detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit has high sensitivity and excellent specificity for detection of Human MSRB2. No significant cross-reactivity or interference between Human MSRB2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MSRB2 (Methionine Sulfoxide Reductase B2) is a Protein Coding gene. Among its related pathways are Protein repair and Metabolism of proteins. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and peptide-methionine (R)-S-oxide reductase activity. An important paralog of this gene is MSRB3. Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance. |
| Gene ID | 22921 |
| Alternative | MSRB2; CBS-1; CBS1; CGI-131; MGC26104; MSRB; PILB; methionine-r-sulfoxide reductase B2 variant 1; methionine-r-sulfoxide reductase B2 variant 3; pilin-like transcription factor |
| Accession | Q9Y3D2 |
Fig.1. Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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