| Product name |
Human IL-3 protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from human IL-3 protein (NP_000579.2, Met1-Phe152) was expressed with a polyhistidine tag at the C-terminus. |
| Activity |
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 6.87ng/mL. |
| Protein length |
The recombinant human IL-3 protein consists of 140 amino acids and predicts a molecular mass of 16 kD.The apparent molecular mass of the human IL-3 is approximately 17-35 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method |
HEK 293 Cells |
| Purity |
>95% as determined by SDS-PAGE |
| Alternative |
Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor |
| Formulation |
Lyophilized from sterile PBS, pH 7.4. |
| Features & Benefits |
Endotoxin: < 1 EU per µg of the protein as determined by the LAL method. |
| Molecular weight |
16kDa |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human IL-3 protein in the buffer which we provide not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| Storage instructions |
Lyophilized Human IL-3 protein, His tag (Animal-Free)should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.118% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping |
The product is shipped at ambient temperature. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
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