| Product name | Human IL-1α Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IL-1α (Ser113-Ala271)(P01583) was expressed with 6×His tag at the C-terminus. |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce D10.G4.1 cells proliferation.The ED₅₀ for this effect is <10 pg/mL.The specific activity of recombinant human IL-1 alpha is approximately >1 x10⁸ IU/ mg. |
| Protein length | The recombinant Human IL-1α Protein consists of 159 amino acids and predicts a molecular mass of 19 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 19 kDa |
| Alternative | Hematopoietin-1, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte |
| Formulation | Lyophilized from sterile PBS，pH 8.0. |
| Features & Benefits | <0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 19 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-1 alpha (IL1 alpha or IL1α) is a member of the interleukin-1 cytokine family, found constitutively present in epithelial layers of the entire gastrointestinal tract, lung, liver, kidney, endothelial cells, and astrocytes. The synthesized IL-1 alpha is a 31 kDa inactive precursor and can be cleaved by intracellular caspase-1 or extracellular proteases to generate the bioactive 17 kDa form and the 16 kDa N-terminal cleavage product. Both precursor and mature IL-1 alpha protein bind to the IL-1 receptor (IL-1R), initiating a cascade of inflammatory cytokines and chemokines production such as IL-6, IL-8, and TNF, in response to viral and bacterial pathogens conditions. IL-1 alpha plays a central role in immune-surveillance mechanisms, stimulating macrophages, neutrophils, and CD8+ T cells activity. |
| Gene ID | 3552 |
| Alternative | Hematopoietin-1, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte |
| Accession | P01583 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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