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Human Glucose transporter 1 (GLUT1) ELISA Kit

Human Glucose transporter 1 (GLUT1) ELISA Kit

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Specification

Product name Human Glucose transporter 1 (GLUT1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Glucose transporter 1 (GLUT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A1 in samples. An antibody specific for SLC5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC5A1; RP1-127L4.1; D22S675; NAGT; SGLT1; Human Na+/glucose cotransporter 1 mRNA; complete cds; sodium/glucose cotransporter 1; solute carrier family 5 (sodium/glucose transporter); member 1; solute carrier f

Product Properties

Kit components • Human Glucose transporter 1 microplate
• Human Glucose transporter 1 standard
• Human Glucose transporter 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Glucose transporter 1 (GLUT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC5A1. No significant cross-reactivity or interference between Human SLC5A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells.GLUT1 is responsible for the low-level of basal glucose uptake required to sustain respiration in all cells. Expression levels of GLUT1 in cell membranes are increased by reduced glucose levels and decreased by increased glucose levels. GLUT1 is also a major receptor for take-up of Vitamin C as well as glucose, especially in non vitamin C producing mammals as part of an adaptation to compensate by participating in a Vitamin C recycling process. In mammals that do produce Vitamin C, GLUT4 is often expressed instead of GLUT1.GLUT1 behaves as a Michaelis-Menten enzyme and contains 12 membrane-spanning alpha helices, each containing 20 amino acid residues.
Gene ID 6523
Alternative SLC5A1; RP1-127L4.1; D22S675; NAGT; SGLT1; Human Na+/glucose cotransporter 1 mRNA; complete cds; sodium/glucose cotransporter 1; solute carrier family 5 (sodium/glucose transporter); member 1; solute carrier f
Accession P13866

Image & description

Fig.1. Human Glucose transporter 1 (GLUT1) Standard Curve.

Fig.1. Human Glucose transporter 1 (GLUT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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