| Product name | Human CD28 protein, Fc tag (Animal-Free) |
| Sequence | Amino acid sequence derived from human CD28 protein (P10747-1, Asn19-Pro152) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Protein length | The recombinant human CD28 protein consists of 371 amino acids and predicts a molecular mass of 42 kD. The apparent molecular mass of the human CD28 is approximately 53-70 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method | HEK 293 Cells |
| Purity | >94% as determined by SDS-PAGE |
| Alternative | CD28 Molecule;T-Cell-Specific Surface Glycoprotein CD28;CD28 Antigen;T-Cell-Specific Surface Glycoprotein;CD28 Antigen (Tp44);Tp44;CD28;TP44 |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.4. |
| Features & Benefits | Endotoxin: <0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 42 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD28 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human CD28 protein, Fc tag (Animal-Free)should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.110% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The cluster of differentiation (CD) antigen CD28 is a 44 kDa, disulphide-bonded, homodimeric glycoprotein, which is a costimulatory molecule expressed on the surface of T lymphocytes and plays an important role in the activation of T cells. It mediates the co stimulation of T cells by binding to B7 molecules on APC (antigen presenting cells) . CD28 signals play a key role in many T cell processes, including cytoskeletal remodeling, production of cytokines, survival, and differentiation. Blocking CD28/B7 interactions may be useful in preventing unwanted activation in allergy and autoimmune diseases, whereas enhancing this interaction can promote tumour rejection. Thus, CD28 and its signalling pathways may prove to be useful targets in the development of new therapeutic treatments. |
| Gene ID | 940 |
| Alternative | CD28 Molecule;T-Cell-Specific Surface Glycoprotein CD28;CD28 Antigen;T-Cell-Specific Surface Glycoprotein;CD28 Antigen (Tp44);Tp44;CD28;TP44 |
| Accession | P10747 |
Fig.SDS-PAGE analysis of Human CD28 protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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