Login Register
English
0

Cart

$ 0

Human Carbohydrate antigen 125 (CA125) ELISA Kit

Human Carbohydrate antigen 125 (CA125) ELISA Kit

Views(146) Publications(0) Catalog no(KTE61460)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Carbohydrate antigen 125 (CA125) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Carbohydrate antigen 125 (CA125) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC16 in samples. An antibody specific for MUC16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MUC16; CA125; FLJ14303; CA125 ovarian cancer antigen; mucin 16

Product Properties

Kit components • Human Carbohydrate antigen 125 microplate
• Human Carbohydrate antigen 125 standard
• Human Carbohydrate antigen 125 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Carbohydrate antigen 125 (CA125) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC16. No significant cross-reactivity or interference between Human MUC16 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CA125 encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.
Gene ID 94025
Alternative MUC16; CA125; FLJ14303; CA125 ovarian cancer antigen; mucin 16
Accession Q8WXI7

Image & description

Fig.1. Human Carbohydrate antigen 125 (CA125) Standard Curve.

Fig.1. Human Carbohydrate antigen 125 (CA125) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Carbohydrate antigen 125 (CA125) ELISA Kit”