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Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) ELISA Kit

Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) ELISA Kit

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Specification

Product name Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) ELISA Kit employs a two-site sandwich ELISA to quantitate ASPH in samples. An antibody specific for ASPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ASPH; AAH; BAH; CASQ2BP1; HAAH; JCTN; junctin; A beta H-J-J; aspartyl/asparaginyl-beta-hydroxylase; cardiac junctin; humbug; junctate; peptide-aspartate beta-dioxygenase

Product Properties

Kit components • Human Aspartyl/asparaginyl beta-hydroxylase microplate
• Human Aspartyl/asparaginyl beta-hydroxylase standard
• Human Aspartyl/asparaginyl beta-hydroxylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ASPH. No significant cross-reactivity or interference between Human ASPH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ASPH is is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum.
Gene ID 444
Alternative ASPH; AAH; BAH; CASQ2BP1; HAAH; JCTN; junctin; A beta H-J-J; aspartyl/asparaginyl-beta-hydroxylase; cardiac junctin; humbug; junctate; peptide-aspartate beta-dioxygenase
Accession Q12797

Image & description

Fig.1. Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) Standard Curve.

Fig.1. Human Aspartyl/asparaginyl beta-hydroxylase (ASPH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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