| Product name | Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit employs a two-site sandwich ELISA to quantitate NARS in samples. An antibody specific for NARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NARS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NARS bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | NARS; ASNRS; NARS1; asparagine tRNA ligase 1; cytoplasmic |
| Kit components | • Human Asparaginyl-tRNA synthetase, cytoplasmic microplate • Human Asparaginyl-tRNA synthetase, cytoplasmic standard • Human Asparaginyl-tRNA synthetase, cytoplasmic detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit has high sensitivity and excellent specificity for detection of Human NARS. No significant cross-reactivity or interference between Human NARS and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. |
| Gene ID | 4677 |
| Alternative | NARS; ASNRS; NARS1; asparagine tRNA ligase 1; cytoplasmic |
| Accession | O43776 |
Fig.1. Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +86-27-59716789
Fax： +86-27-59716788
Email： service@abbkine.com
Address： Bldg C17, Optics Valley International Biomedicine Park, Wuhan, China. 430223
Reviews
There are no reviews yet.