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Human Alcohol dehydrogenase (ADH) ELISA Kit

Human Alcohol dehydrogenase (ADH) ELISA Kit

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Specification

Product name Human Alcohol dehydrogenase (ADH) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Alcohol dehydrogenase (ADH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1A in samples. An antibody specific for ADH1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADH1A; ADH1; ADH; alpha subunit; alcohol dehydrogenase 1 (class I); alpha polypeptide; aldehyde reductase; class I alcohol dehydrogenase; alpha subunit

Product Properties

Kit components • Human Alcohol dehydrogenase microplate
• Human Alcohol dehydrogenase standard
• Human Alcohol dehydrogenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Alcohol dehydrogenase (ADH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADH1A. No significant cross-reactivity or interference between Human ADH1A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Arginine vasopressin is a hormone found in most mammals, including humans. Vasopressin is a peptide hormone. It is derived from a preprohormone precursor that is synthesized in the hypothalamus and stored in vesicles at the posterior pituitary. Most of it is stored in the posterior pituitary to be released into the blood stream; however, some of it is also released directly into the brain.One of the most important roles of AVP is to regulate the body's retention of water; it is released when the body is dehydrated and causes the kidneys to conserve water, thus concentrating the urine, and reducing urine volume. In high concentrations, it also raises blood pressure by inducing moderate vasoconstriction. In addition, it has a variety of neurological effects on the brain, having been found, for example, to influence pair-bonding in voles.
Gene ID 124
Alternative ADH1A; ADH1; ADH; alpha subunit; alcohol dehydrogenase 1 (class I); alpha polypeptide; aldehyde reductase; class I alcohol dehydrogenase; alpha subunit
Accession P07327

Image & description

Fig.1. Human Alcohol dehydrogenase (ADH) Standard Curve.

Fig.1. Human Alcohol dehydrogenase (ADH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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