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Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit

Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit

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Specification

Product name Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit employs a two-site sandwich ELISA to quantitate AGMAT in samples. An antibody specific for AGMAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGMAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGMAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGMAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative AGMAT; FLJ23384; agmatinase

Product Properties

Kit components • Human Agmatine Ureohydrolase/Agmatinase microplate
• Human Agmatine Ureohydrolase/Agmatinase standard
• Human Agmatine Ureohydrolase/Agmatinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGMAT. No significant cross-reactivity or interference between Human AGMAT and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background In humans, Agmatinase is encoded by the AGMAT gene. In enzymology, an agmatinase is an enzyme that catalyzes the chemical reaction This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is agmatine amidinohydrolase. Other names in common use include agmatine ureohydrolase, and SpeB. This enzyme participates in urea cycle and metabolism of amino groups.
Gene ID 79814
Alternative AGMAT; FLJ23384; agmatinase
Accession Q9BSE5

Image & description

Fig.1. Human Agmatine Ureohydrolase/Agmatinase (AGMAT) Standard Curve.

Fig.1. Human Agmatine Ureohydrolase/Agmatinase (AGMAT) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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