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Human Afamin (AFM) ELISA Kit

Human Afamin (AFM) ELISA Kit

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Specification

Product name Human Afamin (AFM) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Afamin (AFM) ELISA Kit employs a two-site sandwich ELISA to quantitate AFM in samples. An antibody specific for AFM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative AFM; ALB2; ALBA; ALF; MGC125338; MGC125339; alpha-albumin

Product Properties

Kit components • Human Afamin microplate
• Human Afamin standard
• Human Afamin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Afamin (AFM) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFM. No significant cross-reactivity or interference between Human AFM and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Afamin is a member of the albumin gene family, which comprises four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. The 'new' gene, called alpha-albumin, was located 10 kb downstream from the AFP locus. The gene is selectively expressed in the liver at late stages of development. The mRNA sequence encodes a predicted secreted protein with the typical triple domain disulfide cross-linked structure. Comparisons of coding and promoter sequences suggested that ALBA could be a phylogenetic intermediate between the ALB and AFP genes.
Gene ID 173
Alternative AFM; ALB2; ALBA; ALF; MGC125338; MGC125339; alpha-albumin
Accession P43652

Image & description

Fig.1. Human Afamin (AFM) Standard Curve.

Fig.1. Human Afamin (AFM) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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