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Human Adrenomedullin (ADM) ELISA Kit

Human Adrenomedullin (ADM) ELISA Kit

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Specification

Product name Human Adrenomedullin (ADM) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Adrenomedullin (ADM) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM in samples. An antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADM; AM; preproadrenomedullin

Product Properties

Kit components • Human Adrenomedullin microplate
• Human Adrenomedullin standard
• Human Adrenomedullin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Adrenomedullin (ADM) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADM. No significant cross-reactivity or interference between Human ADM and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Adrenomedullin (ADM) is a peptide associated with pheochromocytoma. Adrenomedullin (AM) is a ubiquitously expressed peptide initially isolated from phaechromyctoma. Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. The human AM gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns.
Gene ID 133
Alternative ADM; AM; preproadrenomedullin
Accession P35318

Image & description

Fig.1. Human Adrenomedullin (ADM) Standard Curve.

Fig.1. Human Adrenomedullin (ADM) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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