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Human Spectrin beta chain, erythrocyte (SPTB) ELISA Kit

Human Spectrin beta chain, erythrocyte (SPTB) ELISA Kit

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Specification

Product name Human Spectrin beta chain, erythrocyte (SPTB) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Spectrin beta chain, erythrocyte (SPTB) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTB in samples. An antibody specific for SPTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPTB; EL3; HS2; HSPTB1; SPH2; membrane cytoskeletal protein; spectrin beta

Product Properties

Kit components • Human Spectrin beta chain, erythrocyte microplate
• Human Spectrin beta chain, erythrocyte standard
• Human Spectrin beta chain, erythrocyte detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Spectrin beta chain, erythrocyte (SPTB) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPTB. No significant cross-reactivity or interference between Human SPTB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Spectrin is an actin crosslinking and molecular scaffold protein that links the cell membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTbN4 is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. Abundantly expressed in brain and pancreatic islets.
Gene ID 6710
Alternative SPTB; EL3; HS2; HSPTB1; SPH2; membrane cytoskeletal protein; spectrin beta
Accession P11277

Image & description

Fig.1. Human Spectrin beta chain, erythrocyte (SPTB) Standard Curve.

Fig.1. Human Spectrin beta chain, erythrocyte (SPTB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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