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HSP 90 (Acetyl Lys292/284) Polyclonal Antibody

HSP 90 (Acetyl Lys292/284) Polyclonal Antibody

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Specification

Product name HSP 90 (Acetyl Lys292/284) Polyclonal Antibody
Immunogen Synthesized peptide derived from the human HSP 90 around the acetylation site of K292/284
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HSP90AA1; HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Renal carcinoma antigen NY-REN-38; HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HSP90AB1 encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. HSP90AB1 encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
Gene ID 3326
Alternative HSP90AA1; HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Renal carcinoma antigen NY-REN-38; HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84
Others Acetyl-HSP 90 (K292/284) Polyclonal Antibody detects endogenous levels of HSP 90 protein only when acetylated at K292/284.
Accession P07900
Observed Band(KD) 85

Image & description

Fig.1. Western Blot analysis of K562 cells using Acetyl-HSP 90 (K292/284) Polyclonal Antibody. Secondary Antibody  was diluted at 1:20000.

Fig.1. Western Blot analysis of K562 cells using Acetyl-HSP 90 (K292/284) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of K562 cells using Acetyl-HSP 90 (K292/284) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of K562 cells using Acetyl-HSP 90 (K292/284) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

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