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Ezrin Polyclonal Antibody

Ezrin Polyclonal Antibody

Views(15) Publications(0) Catalog no(ABP51314)
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Specification

Product name Ezrin Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Ezrin at AA range: 480-560
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The cytoplasmic peripheral membrane protein encoded by EZR functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for EZR. Alternatively spliced variants have also been described for EZR.
Gene ID 7430
Alternative EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81
Others Ezrin Polyclonal Antibody detects endogenous levels of Ezrin protein.
Accession P15311
Observed Band(KD) 70

Image & description

Fig.1. Western Blot analysis of various cells using Ezrin Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using Ezrin Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of MCF7 cells using Ezrin Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of MCF7 cells using Ezrin Polyclonal Antibody diluted at 1:2000.

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