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HNF-4α Polyclonal Antibody

HNF-4α Polyclonal Antibody

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Specification

Product name HNF-4α Polyclonal Antibody
Immunogen Synthesized peptide derived from human HNF4-α around the non-phosphorylation site of S313
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Hepatocyte nuclear factor 4-alpha encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. Hepatocyte nuclear factor 4-alpha controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.
Gene ID 3172
Alternative HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4
Others HNF-4α Polyclonal Antibody detects endogenous levels of HNF-4α protein.
Accession P41235
Observed Band(KD) 52

Image & description

Fig. Western Blot analysis of various cells using HNF-4α Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of various cells using HNF-4α Polyclonal Antibody diluted at 1:2000.

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