| Product name | Gremlin-2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human Gremlin-2 protein at amino acid sequence of 71-120 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | ELISA, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:500-1:200), ELISA (1:10000-1:20000). |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen |
| Alternative | Gremlin-2; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; Protein related to DAN and cerberus |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Gremlin-2 encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which Gremlin-2 belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, Gremlin-2 may play a role in regulating organogenesis, body patterning, and tissue differentiation. |
| Gene ID | 64388 |
| Alternative | Gremlin-2; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; Protein related to DAN and cerberus |
| Others | The antibody detects endogenous levels of Gremlin-2 |
| Accession | Q9H772 |
| Observed Band(KD) | 19 |
Fig.1. Western blot analysis of RAT-brain lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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