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PFK-C Polyclonal Antibody

PFK-C Polyclonal Antibody

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Specification

Product name PFK-C Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human PFK-C at AA range: 310-390
Host Rabbit
Reactivity Human, Monkey, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase, EC 2. . 1). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.
Gene ID 5214
Alternative PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase
Others PFK-C Polyclonal Antibody detects endogenous levels of PFK-C protein.
Accession Q01813
Observed Band(KD) 84

Image & description

Fig.1. Western Blot analysis of various cells using PFK-C Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using PFK-C Polyclonal Antibody.

Fig.2. Western Blot analysis of HUVEC cells using PFK-C Polyclonal Antibody.

Fig.2. Western Blot analysis of HUVEC cells using PFK-C Polyclonal Antibody.

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