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GDI-2 Polyclonal Antibody

GDI-2 Polyclonal Antibody

Views(14) Publications(0) Catalog no(ABP53048)
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Specification

Product name GDI-2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human GDI-2
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative GDI2; RABGDIB; Rab GDP dissociation inhibitor beta; Rab GDI beta; Guanosine diphosphate dissociation inhibitor 2; GDI-2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 2665
Alternative GDI2; RABGDIB; Rab GDP dissociation inhibitor beta; Rab GDI beta; Guanosine diphosphate dissociation inhibitor 2; GDI-2
Others GDI-2 Polyclonal Antibody detects endogenous levels of GDI-2 protein.
Accession P50395
Observed Band(KD) 50

Image & description

Fig.1. Western Blot analysis of A549, HT29, K562,  Mouse kidney, Mouse lung cells using GDI-2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of A549, HT29, K562, Mouse kidney, Mouse lung cells using GDI-2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

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