|Product name||GAP-43 Monoclonal Antibody|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:200-1:500).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen|
|Molecular weight||38, 43 KD|
|Storage buffer||PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The protein encoded by GAP43 (growth associated protein 43) has been termed a growth or plasticity protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for GAP43.|
|Others||The antibody detects endogenous GAP-43 protein.|
Fig.1. Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue using GAP-43 Monoclonal Antibody.
Fig.2. Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using GAP-43 Monoclonal Antibody.