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GAL4 DNA Binding Domain Polyclonal Antibody

GAL4 DNA Binding Domain Polyclonal Antibody

Views(654) Publications(0) Catalog no(ABP57232)
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Specification

Product name GAL4 DNA Binding Domain Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Human
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:50000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
Others The antibody detects endogenous GAL4 DNA Binding Domain protein.

Image & description

Fig. Western blot analysis of Recombinant BD protein using GAL4 DNA Binding Domain Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western blot analysis of Recombinant BD protein using GAL4 DNA Binding Domain Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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