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Human Semaphorin-4A (SEMA4A) ELISA Kit

Human Semaphorin-4A (SEMA4A) ELISA Kit

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Specification

Product name Human Semaphorin-4A (SEMA4A) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Semaphorin-4A (SEMA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4A in samples. An antibody specific for SEMA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SEMA4A; RP11-54H19.2; CORD10; FLJ12287; RP35; SEMAB; SEMB; OTTHUMP00000015917; OTTHUMP00000015918; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4A; semaphori

Product Properties

Kit components • Human Semaphorin-4A microplate
• Human Semaphorin-4A standard
• Human Semaphorin-4A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Semaphorin-4A (SEMA4A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA4A. No significant cross-reactivity or interference between Human SEMA4A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.Mice treated with Sema4a had enhanced priming of antigen-specific T cells secreting both IL4 and gamma-interferon (IFNG). Treatment of mice with anti-Sema4a early after immunization with myelin oligodendrocyte glycoprotein suppressed the development of experimental autoimmune encephalomyelitis. In these mice, infiltration of mononuclear inflammatory cells was ablated by inhibition of the generation of antigen-specific T cells by anti-Sema4a. Expression cloning determined that the high-affinity receptor for Sema4a is Tim2. Sema4a stimulation of cells expressing Tim2 induced tyrosine phosphorylation of Tim2 but not of Tim3.
Gene ID 64218
Alternative SEMA4A; RP11-54H19.2; CORD10; FLJ12287; RP35; SEMAB; SEMB; OTTHUMP00000015917; OTTHUMP00000015918; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4A; semaphori
Accession Q9H3S1

Image & description

Fig.1. Human Semaphorin-4A (SEMA4A) Standard Curve.

Fig.1. Human Semaphorin-4A (SEMA4A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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