| Product name | Fli-1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Fli-1 at AA range: 260-340 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | FLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 50kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | FLI1 encodes friend leukemia integration 1 transcription factor containing an ETS DNA-binding domain. FLI1 can undergo a t (11;22) (q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4;11) (q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. |
| Gene ID | 2313 |
| Alternative | FLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB |
| Others | Fli-1 Polyclonal Antibody detects endogenous levels of Fli-1 protein. |
| Accession | Q01543 |
| Observed Band(KD) | 50 |
Fig.1. Western Blot analysis of various cells using Fli-1 Polyclonal Antibody.
Fig.2. Western Blot analysis of 3T3 cells using Fli-1 Polyclonal Antibody.
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