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CA VB Polyclonal Antibody

CA VB Polyclonal Antibody

Views(52) Publications(0) Catalog no(ABP53624)
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Specification

Product name CA VB Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human CA VB at AA rangle: 210-290
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CA5B; Carbonic anhydrase 5B; mitochondrial; Carbonate dehydratase VB; Carbonic anhydrase VB; CA-VB

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
Gene ID 11238
Alternative CA5B; Carbonic anhydrase 5B; mitochondrial; Carbonate dehydratase VB; Carbonic anhydrase VB; CA-VB
Others CA VB Polyclonal Antibody detects endogenous levels of CA VB protein.
Accession Q9Y2D0
Observed Band(KD) 38

Image & description

Fig. Western Blot analysis of 3T3 cells using CA VB Polyclonal Antibody.

Fig. Western Blot analysis of 3T3 cells using CA VB Polyclonal Antibody.

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