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FITC, Goat Anti-Mouse IgG

Product Information

  • Product name

    FITC, Goat Anti-Mouse IgG

  • Immunogen

    Mouse IgG whole molecule

  • Host

    Goat

  • Reactivity

    Mouse

  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested 1:50-1:1000 for most fluorescent applications.

  • Clonality

    Polyclonal

  • Isotype

    Goat IgG

  • Purification

    Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE

  • Conjugate

    FITC

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

  • Others

    The antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

  1. In vitro observation: the GFP-E. coli adhering to porcine erythrocytes can be removed by porcine alveolar macrophages

    W Yin, C Wang, K Fan, N Sun, Y Sun, H Li. PeerJ, 2019.

  2. STING recruits NLRP3 to the ER and deubiquitinates NLRP3 to activate the inflammasome

    Wenbiao Wang, Dingwen Hu, Yuqian Feng, et al.

  3. 3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition

    Xuanzhi Wang, Xingliang Dai, Xinzhi Zhang, et al. J Biomed Mater Res A. 2019 Feb;107(2):383-391.

  4. In vitro observation: the GFP-E. coliadhering to porcine erythrocytes can be removed by porcine alveolar macrophages

    Wei Yin, Chun Wang, Kuohai Fan, et al. PeerJ. 2019 Mar 8;7:e6439.

  5. IFN-γ aggravates neointimal hyperplasia by inducing endoplasmic reticulum stress and apoptosis in macrophages by promoting ubiquitin-dependent liver X receptor-α degradation

    Zhao Q, Zhou D, You H, et al. FASEB J, 2017, 31(12): 5321-5331.

  6. The Therapeutic Effect of Dexmedetomidine on Rat Diabetic Neuropathy Pain and the Mechanism

    Lu Y, Lin B, Zhong J, et al. Biological & Pharmaceutical Bulletin , 2017, 40(9), 1432-1438.

  7. Comparative analysis of two ferritin subunits from blunt snout bream (Megalobrama amblycephala): Characterization, expression, iron depriving and bacteriostatic activity

    Ding, Zhujin, et al. Fish & Shellfish Immunology (2017).

  8. Mechanisms responsible for the inhibitory effects of epothilone B on scar formation after spinal cord injury

    Zhao, Wei, et al. NEURAL REGENERATION RESEARCH 12.3: 478-485.

  9. Mechanisms responsible for the inhibitory effects of epothilone B on scar formation after spinal cord injury

    Zhao, Wei, et al. Neural regeneration research 12.3 (2017): 478.

  10. Cloning the Horse RNA Polymerase I Promoter and Its Application to Studying Influenza Virus Polymerase Activity.

    Lu G, He D, Wang Z, et al. Viruses, 2016, 8(6): 119.

  11. Surface-Displayed IL-10 by Recombinant Lactobacillus plantarum Reduces Th1 Responses of RAW264. 7 Cells Stimulated with Poly (I: C) or LPS.

    Cai R, Jiang Y, Yang W, et al. Journal of microbiology and biotechnology, 2016, 26(2): 421-431.

  12. Preoperative Detection and Intraoperative Visualization of Brain Tumors for More Precise Surgery: A New Dual‐Modality MRI and NIR Nanoprobe.

    Li C, Cao L, Zhang Y, et al. Small, 2015, 11(35): 4517-4525.

  13. Curcumin induces M2 macrophage polarization by secretion IL-4 and/or IL-13.

    Gao S, Zhou J, Liu N, et al. Journal of molecular and cellular cardiology, 2015, 85: 131-139.

  14. β-Thymosins participate in antiviral immunity of red swamp crayfish (Procambarus clarkii).

    Shi X Z, Shi L J, Zhao Y R, et al. Developmental & Comparative Immunology, 2015, 51(2): 213-225.

Cat #: A22110

  • Size
  • Price
  • 100μl $25
  • 500μl $100

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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