|Product name||ExKine™ Pro Total Protein Extraction Kit for Plant Tissues|
|Applications notes||Abbkine's ExKine™ Pro Total Protein Extraction Kit for Plant Tissues provides a simple and convenient method for extracting total protein from plant tissues.|
|Kit components||• Denaturing Cell Lysis Buffer
• Protein Extraction Filter Cartridge
• Collection Tube with Cap
• Plastic Rod
|Features & Benefits||• Simple and fast, it can be extracted in 8 min;
• Without altered protein profile, high yield (2-8 mg/mL);
• No need for liquid nitrogen grinding, optimized lysis buffer for more efficient protein extraction;
• Applicable samples are complete, including plant tissue, leaves, seeds, soft stems, and roots;
• Apply in various downstream assays, including Western blotting, SDS-PAGE, etc.
|Usage notes||1．Protease inhibitors are not necessarily added, but if downstream experiments take longer or are preserved for longer periods after protein extraction, it is advisable to add protease inhibitors. Protein concentration was determined by BCA method, but not by Bradford method. To study protein phosphorylation, phosphatase inhibitors should be added to the lysis buffer before use.
2．Before WB, still mix the sample and loading buffer to boiling.
3．The Plastic Rod is reusable, rinse thoroughly with deionized water, and dry it with paper towel.
4．If the protein concentration is low, increase the weight of tissues or reduce the volume of Denaturing Cell Lysis Buffer.
5．Partial tissues that is not completely lysed will not affect the quality of the sample.
|Storage instructions||Stored at room temperature for 12 months.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||ExKine™ Pro Plant Tissue Total Protein Extraction Kit (column method) is a new generation of ultra-fast protein extraction tool. More and more evidences show that the most commonly used RIPA buffer may lead to random loss of protein, resulting in many difficult data that are difficult to explain. ExKine™ Pro Total Protein Extraction Kit uses centrifugal column extraction technology combined with optimized lysis buffer to extract total protein more quickly and effectively, without grinding in liquid nitrogen, making WB results more accurate.|
Fig 1. Total proteins were extracted from different plants (Oryza, Arabidopsis). Lane left, proteins extracted by Abbkine Assay Kits. Lane right, proteins extracted by B-brand Assay Kits. After blocking, incubate with primary antibody (A01050, 1:5000), incubate secondary antibody (A21010，1:10000), Chemiluminescence (BMU101-EN).
Fig 2. Total proteins were extracted from different plants (Arabidopsis, Soybeans). Lane left, proteins extracted by Abbkine Assay Kits. Lane right, proteins extracted by B-brand Assay Kits. After blocking, incubate with primary antibody (A01070, 1:5000), incubate secondary antibody (A21010，1:10000), Chemiluminescence (BMU101-EN).
Fig 3. Total proteins were extracted from different plants (Oryza, Arabidopsis). Lane left, proteins extracted by Abbkine Assay Kits. Lane right, proteins extracted by B-brand Assay Kits. After blocking, incubate with primary antibody (A01110, 1:5000), incubate secondary antibody (A21010，1:10000), Chemiluminescence (BMU101-EN).
Fig 4. Plant protein profiles extracted by denaturing cell lysis buffer (Abbkine KTP3008). Extracted proteins were separated in 12% SDS-PAGE and stained with coomassie blue (BMU105-EN). Lane 1, soybean; lane 2, maize; lane 3, spinach; lane 4, Arabidopsis.
Author：Zhao, Kunkun, et al. Publication name：Plant Biotechnology Journal IF：13.8
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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