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Human Synaptotagmin-12 (SYT12) ELISA Kit

Human Synaptotagmin-12 (SYT12) ELISA Kit

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Specification

Product name Human Synaptotagmin-12 (SYT12) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12 in samples. An antibody specific for SYT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SYT12; SRG1; SYT11;

Product Properties

Kit components • Human Synaptotagmin-12 microplate
• Human Synaptotagmin-12 standard
• Human Synaptotagmin-12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Synaptotagmin-12 (SYT12) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT12. No significant cross-reactivity or interference between Human SYT12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Syt12 colocalises with and binds Syt1 on synaptic vesicles, but regulates spontaneous release independently from Syt1. Syt12 is phosphorylated by cAMP-dependent protein kinase A (PKA) at a single site, and mutation of this site blocks the effect of Syt12 on spontaneous release. Syt12 is not essential for basal synaptic transmission, but is required for cAMP-dependent mossy fibre long-term potentiation (LTP), a presynaptic form of long-term plasticity that is induced by PKA activation
Gene ID 91683
Alternative SYT12; SRG1; SYT11;
Accession Q8IV01

Image & description

Fig.1. Human Synaptotagmin-12 (SYT12) Standard Curve.

Fig.1. Human Synaptotagmin-12 (SYT12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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