|Product name||ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues|
|Applications notes||ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues is composed of rapid and efficient solutions for lysis of mammalian adherent cells, nonadherent cells, and tissues, which is compatible with many different applications, such as reporter assays, protein purification, Western blot, immunoprecipitation.|
|Kit components||• Denaturing cell lysis buffer
• Native cell lysis buffer
• Protein extraction filter cartridges
• Collection tubes with cap
• Plastic rods
|Features & Benefits||• Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
• Fast and convenient—non-denatured, active proteins are purified in less than 8 min.
• Compatible—apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.
|Usage notes||Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.|
|Storage instructions||Stored at room temperature for 12 months.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The preparation of extracts from animal cells or tissues.The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins.|
Fig.1 Protein profiles of mouse liver extracted by denatureing lysis buffer and native buffer from different manufacturers. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, Marker; Lane 2, native buffer from A manufacturer; Lane 3, native buffer from Abbkine; Lane 4, denatureing lysis buffer from A manufacturer; Lane 5, denatureing lysis buffer from B manufacturer; Lane 6, denatureing lysis buffer from C manufacturer; Lane 7, denatureing lysis buffer from Abbkine; Lane 8, denatureing lysis buffer from D manufacturer.
Fig.2 Total proteins were extracted from 293 cells and mouse liver. Lane 1, 5 μL proteins extracted by B assay kits; Lane 2, 2.5 μL proteins extracted by B assay kits; Lane 3, 5 μL proteins extracted by Abbkine assay kits; Lane 4, 2.5 μL proteins extracted by Abbkine assay kits.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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