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Human Tropomodulin-3 (TMOD3) ELISA Kit

Human Tropomodulin-3 (TMOD3) ELISA Kit

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Product name Human Tropomodulin-3 (TMOD3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tropomodulin-3 (TMOD3) ELISA Kit employs a two-site sandwich ELISA to quantitate TMOD3 in samples. An antibody specific for TMOD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMOD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMOD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMOD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMOD3; UTMOD;

Product Properties

Kit components • Human Tropomodulin-3 microplate
• Human Tropomodulin-3 standard
• Human Tropomodulin-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tropomodulin-3 (TMOD3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMOD3. No significant cross-reactivity or interference between Human TMOD3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background By RNA dot blot analysis, Conley et al. (2001) confirmed expression of TMOD3, which they designated UTMOD, in all adult and fetal tissues examined. Expression was relatively uniform in all tissues tested, showing less than a 4-fold difference between the highest and lowest levels. Using EST database searches, sequencing, and RT-PCR, Cox and Zoghbi (2000) cloned human and mouse cDNAs corresponding to the TMOD3 gene. Human TMOD3 encodes a deduced 352-amino acid protein. Northern blot analysis on human tissues detected ubiquitous expression of 7 TMOD3 transcripts ranging in size from approximately 1 to 9.5 kb. In the mouse, Tmod3 expression was present as early as embryonic day 7 and continued throughout development.
Gene ID 29766
Alternative TMOD3; UTMOD;
Accession Q9NYL9

Image & description

Fig.1. Human Tropomodulin-3 (TMOD3) Standard Curve.

Fig.1. Human Tropomodulin-3 (TMOD3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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