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ExKine™ Cytoplasmic Protein Extraction Kit

ExKine™ Cytoplasmic Protein Extraction Kit

Views(320) Publications(2) Catalog no(KTP3003)
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Specification

Product name ExKine™ Cytoplasmic Protein Extraction Kit
Applications notes Abbkine ExKine™ Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic extracts from mammalian cultured cells or tissues. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the nuclear fraction is removed. Non-denatured, active proteins are purified in less than two hours.

Product Properties

Kit components • Cytoplasmic Solution A (CES A)
• Cytoplasmic Solution B (CES B)
• DTT (500X)
• Protease Inhibitor (100X)
Features & Benefits • Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
• Fast and convenient—non-denatured, active proteins are purified in less than two hours.
• Compatible—apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.
Usage notes Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The preparation of an extract from Cytoplasm is often the first step in studying Cytoplasmic proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.

Image & description

Fig. Western blot of specific proteins fraction using Abbkine ExKine™ Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C1: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). C2: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070).

Fig. Western blot of specific proteins fraction using Abbkine ExKine™ Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C1: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). C2: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070).

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