| Product name | ExKine™ Cytoplasmic Protein Extraction Kit |
| Applications notes | Abbkine ExKine™ Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic extracts from mammalian cultured cells or tissues. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the nuclear fraction is removed. Non-denatured, active proteins are purified in less than two hours. |
| Kit components | • Cytoplasmic Solution A (CES A) • Cytoplasmic Solution B (CES B) • DTT (500X) • Protease Inhibitor (100X) |
| Features & Benefits | • Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations. • Fast and convenient—non-denatured, active proteins are purified in less than two hours. • Compatible—apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc. |
| Usage notes | Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The preparation of an extract from Cytoplasm is often the first step in studying Cytoplasmic proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins. |
Fig. Western blot of specific proteins fraction using Abbkine ExKine™ Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C1: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). C2: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070).
Author：Wang Y, Wo Y, Lu T, Sun X, Liu A, Dong Y, Du W, Su W, Huang Z, Jiao W Publication name：Transl Lung Cancer Res IF：5.132
Author：Y Wang, Y Wo, T Lu Publication name：Translational Lung cancer research IF：5.1
Author：Han L, Li D, Hang Y, Zong X, Lv J, Bai X, Lu Y, Zhang P, Zhou M, Wu Z, Hu W Publication name：Front Genet IF：3.258
Author：L Han, D Li, Y Hang, X Zong Publication name：Frontiers in Genetics IF：2.7
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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