Login Register
English
0

Cart

$ 0

Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit

Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit

Views(726) Publications(0) Catalog no(KTE60040)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit employs a two-site sandwich ELISA to quantitate WASF2 in samples. An antibody specific for WASF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWASF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WASF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WASF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative WASF2; SCAR2; WAVE2; dJ393P12.2; IMD2; OTTHUMP00000211879; WASP family Verprolin-homologous protein 2; suppressor of cyclic-AMP receptor (WASP-family)

Product Properties

Kit components • Human Wiskott-Aldrich syndrome protein family member 2 microplate
• Human Wiskott-Aldrich syndrome protein family member 2 standard
• Human Wiskott-Aldrich syndrome protein family member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human WASF2. No significant cross-reactivity or interference between Human WASF2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Wiskott-Aldrich syndrome protein family member 2 is a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X.Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
Gene ID 10163
Alternative WASF2; SCAR2; WAVE2; dJ393P12.2; IMD2; OTTHUMP00000211879; WASP family Verprolin-homologous protein 2; suppressor of cyclic-AMP receptor (WASP-family)
Accession Q9Y6W5

Image & description

Fig.1. Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) Standard Curve.

Fig.1. Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Wiskott-Aldrich syndrome protein family member 2 (WASF2) ELISA Kit”