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ERp57 Polyclonal Antibody

ERp57 Polyclonal Antibody

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Specification

Product name ERp57 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human ERp57
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PDIA3; ERP57; ERP60; GRP58; Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PDIA3 encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. Protein disulfide-isomerase A3was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Gene ID 2923
Alternative PDIA3; ERP57; ERP60; GRP58; Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
Others ERp57 Polyclonal Antibody detects endogenous levels of ERp57 protein.
Accession P30101
Observed Band(KD) 55

Image & description

Fig.1. Western Blot analysis of A549, HT29, K562 cells using ERp57 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of A549, HT29, K562 cells using ERp57 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

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