|Product name||EliKine™ Testosterone ELISA Kit|
|Reactivity||Bovine, Chicken, Dog, Goat, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Swine|
|Applications notes||EliKine™ Testosterone ELISA Kit employs the competitive inhibition enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for testosterone and Horseradish Peroxidase (HRP) conjugated testosterone. The competitive inhibition reaction is launched between with HRP labeled testosterone and unlabeled testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of testosterone in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Other biological fluids, Plasma, Serum|
|Assay type||Competitive ELISA (quantitative)|
|Assay duration||Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.|
|Kit components||• Testosterone microplate
• Testosterone standard
• HRP conjugated testosterone
• Testosterone detect antibody
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
|Features & Benefits||EliKine™ Testosterone ELISA Kit has high sensitivity and excellent specificity for detection of Testosterone. No significant cross-reactivity or interference between Testosterone and analogues was observed.|
|Calibration range||0.1 ng/ml-20 ng/ml|
|Limit of detection||0.1 ng/ml|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for
|Storage instructions||The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Testosterone is the primary male sex hormone and an anabolic steroid. In men, testosterone plays a key role in the development of male reproductive tissues such as the testis and prostate, as well as promoting secondary sexual characteristics such as increased muscle and bone mass, and the growth of body hair. In addition, testosterone is involved in health and well-being, and the prevention of osteoporosis. Insufficient levels of testosterone in men may lead to abnormalities including frailty and bone loss. Testosterone is a steroid from the androstane class containing a keto and hydroxyl groups at the three and seventeen positions respectively. It is biosynthesized in several steps from cholesterol and is converted in the liver to inactive metabolites. It exerts its action through binding to and activation of the androgen receptor.|
Fig.1. Testosterone Standard Curve.
Fig.2. EliKine™ Testosterone ELISA Kit is a competitive ELISA to quantitate Testosterone in samples.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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