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EliKine™ Free Thyroxine (fT4) ELISA Kit

EliKine™ Free Thyroxine (fT4) ELISA Kit

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Product name EliKine™ Free Thyroxine (fT4) ELISA Kit
Reactivity Bovine, Chicken, Dog, Goat, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Swine
Applications ELISA
Applications notes EliKine™ fT4 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT4. A competitive inhibition reaction is launched between fT4 (Standards or samples) and Biotin-conjugated fT4 with the pre-coated antibody specific for fT4. The more amount of fT4 in samples, the less antibody bound by Biotin-conjugated fT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT4 in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Other biological fluids, Plasma, Serum
Assay type Competitive ELISA (quantitative)
Assay duration Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.

Product Properties

Kit components • fT4 microplate
• fT4 standard
• Biotin conjugated fT4
• Avidin-HRP
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ fT4 ELISA Kit has high sensitivity and excellent specificity for detection of fT4. No significant cross-reactivity or interference between fT4 and analogues was observed.
Calibration range 4 pmol/L-64 pmol/L
Limit of detection 4.0 pmol/L
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for
Storage instructions The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Over 99% of Triiodothyronine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T4 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.

Image & description

Fig.1. fT4 Standard Curve.

Fig.1. fT4 Standard Curve.

Fig.2. EliKine™ fT4 ELISA Kit is a competitive ELISA to quantitate fT4 in samples.

Fig.2. EliKine™ fT4 ELISA Kit is a competitive ELISA to quantitate fT4 in samples.

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