| Product name | DNA Ligase III Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human DNA Ligase III at AA range: 110-190 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | LIG3; DNA ligase 3; DNA ligase III; Polydeoxyribonucleotide synthase [ATP] 3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 100kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | LIG3 is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. DNA ligase 3 encoded by LIG3 is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Gene ID | 3980 |
| Alternative | LIG3; DNA ligase 3; DNA ligase III; Polydeoxyribonucleotide synthase [ATP] 3 |
| Others | DNA Ligase III Polyclonal Antibody detects endogenous levels of DNA Ligase III protein. |
| Accession | P49916 |
| Observed Band(KD) | 100 |
Fig.1. Western Blot analysis of various cells using DNA Ligase III Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of MCF7 cells using DNA Ligase III Polyclonal Antibody diluted at 1:1000.
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