|Product name||TraKine™ Mitochondrion and Nuclear Staining Kit|
|Applications notes||Abbkine TraKine™ Mitochondrion and Nuclear Staining Kit provides two stains-Orange fluorescent MitoOrange (Ex/Em = 579/599 nm) and blue-fluorescent Hoechst 33342 (Ex/Em: 350/461 nm, when bound to DNA) for highly selective mitochondrial and nuclear staining, respectively, in live, GFP–transfected cells. Both dyes are retained after formaldehyde fixation and permeabilization. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.|
|Kit components||• MitoOrange™ (1000×)
• Hoechst 33342 (1000×)
• Assay Buffer (10×)
|Features & Benefits||• Enables the uniform staining of mitochondrion and nuclear across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells.
• Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.
• Proprietary MitoOrange™ (Ex/Em = 579/599 nm)-easily permeates intact living cells and can be retained after formaldehyde fixation and permeabilization, suitable for double labeling experiments.
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.|
Fig. Hela cells stained with TraKine™ Mitochondrion and Nuclear Staining Kit.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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