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Cytokeratin 19 Polyclonal Antibody

Cytokeratin 19 Polyclonal Antibody

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Specification

Product name Cytokeratin 19 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cytokeratin 19 at AA range: 200-280
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative KRT19; Keratin; type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 44 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Keratin 19 encoded by KRT19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Gene ID 3880
Alternative KRT19; Keratin; type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19
Others Cytokeratin 19 Polyclonal Antibody detects endogenous levels of Cytokeratin 19 protein.
Accession P08727

Image & description

Fig.1. Immunofluorescence analysis of human liver tissue. 1, Cytokeratin 19 Polyclonal Antibody (red) was diluted at 1:200 (4°,overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.1. Immunofluorescence analysis of human liver tissue. 1, Cytokeratin 19 Polyclonal Antibody (red) was diluted at 1:200 (4°,overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Western Blot analysis of various cells using Cytokeratin 19 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of various cells using Cytokeratin 19 Polyclonal Antibody diluted at 1:1000.

Fig.3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue. 1, CytokeRatin 19 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue. 1, CytokeRatin 19 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, CytokeRatin 19 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, CytokeRatin 19 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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