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Cystatin A Polyclonal Antibody

Cystatin A Polyclonal Antibody

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Specification

Product name Cystatin A Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cystatin A at AA range: 20-100
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CSTA; STF1; STFA; Cystatin-A; Cystatin-AS; Stefin-A

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.
Gene ID 1475
Alternative CSTA; STF1; STFA; Cystatin-A; Cystatin-AS; Stefin-A
Others Cystatin A Polyclonal Antibody detects endogenous levels of Cystatin A protein.
Accession P01040
Observed Band(KD) 11

Image & description

Fig.1. Western Blot analysis of various cells using Cystatin A Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Cystatin A Polyclonal Antibody.

Fig.2. Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody.

Fig.2. Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody.

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