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Cyclin B1 Polyclonal Antibody

Cyclin B1 Polyclonal Antibody

Views(333) Publications(0) Catalog no(ABP57274)
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Specification

Product name Cyclin B1 Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:50-1:200).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Gene ID 891
Others The antibody detects Cyclin B1.
Accession P14635
Observed Band(KD) 55

Image & description

Fig.1. Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using HSP27Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using HSP27Mouse mAb diluted at 1:200.

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